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tcf lef reporter kit  (BPS Bioscience)


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    BPS Bioscience tcf lef reporter kit
    Tcf Lef Reporter Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60500/TCF%2FLEF+Reporter+Kit/pm41963939-106-14-22
    Average 94 stars, based on 46 article reviews
    tcf lef reporter kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Proliferation Assay:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Isolation:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Ligation:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Microarray:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Gene Expression:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Transgenic Assay:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Sequencing:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    In Situ Hybridization:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Polymerase Chain Reaction:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Chromatin Immunoprecipitation:

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA. .. Themo Fisher Scientific Cat# MSS204272 Stealth siRNA for mouse MyD88; UUCAGUAUAUCCUCACGGUCUAACA.

    Transfection:

    Article Title: Mechanistic and functional characterization of ABTB2 as a novel target for pancreatic cancer therapy
    Article Snippet: .. Cells were seeded into 96-well plates at a density of 8 × 10 3 cells per well and transfected with the T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter vector or a negative control reporter vector from the TCF/LEF Reporter Kit (BPS Bioscience, San Diego, CA), according to the manufacturer’s instructions. .. 48 h after transfection, cells were harvested and lysed with the passive lysis buffer provided in the Promega Luciferase System (Promega, Madison, WI).

    Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
    Article Snippet: .. The CMT-93 Piezo1 − /− cell line was transiently transfected using the TCF/LEF Reporter Kit (BPS Biosciences) with Lipofectamine 2000 (Thermo Fisher Scientific), following the manufacturer’s instructions. ..

    Luciferase:

    Article Title: Mechanistic and functional characterization of ABTB2 as a novel target for pancreatic cancer therapy
    Article Snippet: .. Cells were seeded into 96-well plates at a density of 8 × 10 3 cells per well and transfected with the T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter vector or a negative control reporter vector from the TCF/LEF Reporter Kit (BPS Bioscience, San Diego, CA), according to the manufacturer’s instructions. .. 48 h after transfection, cells were harvested and lysed with the passive lysis buffer provided in the Promega Luciferase System (Promega, Madison, WI).

    Article Title: CDK4 inhibition by luteolin enhances lenvatinib sensitivity in HCC via Wnt/β-catenin modulation.
    Article Snippet: 235 Ubiquitination was detected by western blot using anti-ubiquitin antibody (1:1000, 236 #3936, Cell Signaling Technology). .. 237 238 TCF4 luciferase reporter assay 239 β-catenin transcription activity was assessed using the TCF/LEF Reporter Kit (Wnt 240 Signaling Pathway) from BPS Bioscience. ..

    Article Title: Design, multicomponent synthesis, and molecular modelling studies of tetrazole biphenyls as REV-ERBα modulators.
    Article Snippet: The efficient synthesis of biphenyl tetrazole derivatives was achieved through a one-pot multicomponent reaction catalyzed by iron nanoparticles supported on silica (Fe/SiO2).. This green and sustainable approach offers significant advantages over conventional catalytic systems, including high yields (up to 92 %), mild reaction conditions (100 C), and excellent catalyst recyclability.. The methodology involves a Suzuki-Miyaura coupling reaction followed by an in situ [2 + 3] cycloaddition, with dimethylformamide (DMF) as the solvent.

    Negative Control:

    Article Title: Mechanistic and functional characterization of ABTB2 as a novel target for pancreatic cancer therapy
    Article Snippet: .. Cells were seeded into 96-well plates at a density of 8 × 10 3 cells per well and transfected with the T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter vector or a negative control reporter vector from the TCF/LEF Reporter Kit (BPS Bioscience, San Diego, CA), according to the manufacturer’s instructions. .. 48 h after transfection, cells were harvested and lysed with the passive lysis buffer provided in the Promega Luciferase System (Promega, Madison, WI).

    Activity Assay:

    Article Title: WNT3A Upregulates the RIP1/HIF-1α/GDF-15 Pathway to Induce EMT and Thereby Increase Colorectal Cancer Cell Migration and Invasion.
    Article Snippet: .. Wnt/β-catenin signaling activity was assessed using the TCF/LEF Reporter Kit (BPS Bioscience, San Diego, CA, USA) according to the manufacturer's instructions. ..

    Article Title: CDK4 inhibition by luteolin enhances lenvatinib sensitivity in HCC via Wnt/β-catenin modulation.
    Article Snippet: 235 Ubiquitination was detected by western blot using anti-ubiquitin antibody (1:1000, 236 #3936, Cell Signaling Technology). .. 237 238 TCF4 luciferase reporter assay 239 β-catenin transcription activity was assessed using the TCF/LEF Reporter Kit (Wnt 240 Signaling Pathway) from BPS Bioscience. ..

    Article Title: miRNA-195-5p modulates cell proliferation and stemness by targeting the Wnt signalling network in breast cancer
    Article Snippet: 24 h later, the cells were harvested in a passive lysis buffer (Promega), and luciferase activity was measured using a Dual-Luciferase assay kit (Promega) according to the manufacturer's protocol. .. Canonical WNT/β-catenin signalling activity was analysed using a TCF/LEF Reporter kit (BPS Biosciences, USA). ..

    Reporter Assay:

    Article Title: CDK4 inhibition by luteolin enhances lenvatinib sensitivity in HCC via Wnt/β-catenin modulation.
    Article Snippet: 235 Ubiquitination was detected by western blot using anti-ubiquitin antibody (1:1000, 236 #3936, Cell Signaling Technology). .. 237 238 TCF4 luciferase reporter assay 239 β-catenin transcription activity was assessed using the TCF/LEF Reporter Kit (Wnt 240 Signaling Pathway) from BPS Bioscience. ..

    Incubation:

    Article Title: Design, multicomponent synthesis, and molecular modelling studies of tetrazole biphenyls as REV-ERBα modulators.
    Article Snippet: The efficient synthesis of biphenyl tetrazole derivatives was achieved through a one-pot multicomponent reaction catalyzed by iron nanoparticles supported on silica (Fe/SiO2).. This green and sustainable approach offers significant advantages over conventional catalytic systems, including high yields (up to 92 %), mild reaction conditions (100 C), and excellent catalyst recyclability.. The methodology involves a Suzuki-Miyaura coupling reaction followed by an in situ [2 + 3] cycloaddition, with dimethylformamide (DMF) as the solvent.



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